Dissociation of Escherichia coli heat-labile enterotoxin adjuvanticity from ADP-ribosyltransferase activity
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چکیده
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Escherichia coli heat-labile enterotoxin. Ganglioside specificity and ADP-ribosyltransferase activity.
Escherichia coli heat-labile enterotoxin (LT) bound to rat glioma C6 cells that had incorporated ganglioside GMl but not to cells that had taken up gangliosides GM2, GD~, or G D ~ ~ . The same specificity also was observed with choleragen, the enterotoxin of Vihrio cholem. The tryptophanyl fluorescence spectra of LT and its B component differed from those of choleragen and its B component, resp...
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Previous studies of the S1 subunit of pertussis toxin, an NAD(+)-dependent ADP-ribosyltransferase, suggested that a small amino-terminal region of amino acid sequence similarity to the active fragments of both cholera toxin and Escherichia coli heat-labile enterotoxin represents a region containing critical active-site residues that might be involved in the binding of the substrate NAD+. Other ...
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Highly purified, polymyxin-released, low molecular weight Escherichia coli heat-labile enterotoxin (LT) catalyzed the hydrolysis of NAD to ADP-ribose and nicotinamide. This NAD glycohydrolase activity was stimulated by dithiothreitol and was independent of cellular components. Nicotinamide formation was enhanced by arginine methyl ester > d-arginine congruent with l-arginine congruent with guan...
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Heat-labile Escherichia coli enterotoxin (LT) has the innate property of being a strong mucosal immunogen and adjuvant. In the attempt to reduce toxicity and maintain the useful immunological properties, several LT mutants have been produced. Some of these are promising mucosal adjuvants. However, so far, only those that were still toxic maintained full adjuvanticity. In this paper we describe ...
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Takai, Y., Kishimoto, A,, Kawahara, Y., Minakuchi, R.. Sano, K., Kikkawa, U., Mori, T., Yu, B., Kaibuchi, K. & Nishizuka, y. (1981) Adv. Cyclic Nucleotide Res. 14, 301-313 Thomas, D. D. & Knoop, F. C. (1982) J. Inject Dis. 145, 141147 Thomas, D. D. & Knoop, F. C. (1983) J . Infect. Dis. 147,450-459 Turnberg, L. A., Bieberdorf, F. A,, Morawski, S. G . & Fordtran, J. S. (1970) J. Clin. Invest. 49...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1995
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.63.5.1617-1623.1995